The conclusion of this study shows that robusta coffe e (Coffea canephora) extract has the potential as an anti-inflammatory and antioxidant in chickens. Data in the form of quantitative duodenum organ histopathology using haematoxylin and eosin staining and histopathological picture of duodenum were observed using a microscope and analyzed descriptively, test for Nrf2 and HO-1 with flow cytometri the result of robusta coffee (Coffea canephora) extract from lampung had a good effect at a dose of 500 mg / kg body weight against the relative number of Nrf2, HO-1, and histopathological improvement in the duodenum. This study used 48 layer chickens, consisting of 4 treatments and 12 replications consisting of negative controls (healthy chickens without coffee supplementation), T1 (coffee extract dose 500 mg / kg BW), T2 (coffee extract dose 1000 mg / kg BW) and T3 (coffee extract dose 1500 mg / kg body weight. Green coffee extract is made from roasted coffee beans. Robusta coffee (Coffea canephora) is a type of coffee with high levels of chlorogenic acid and high caffeine content which has the potential as an antioxidant so that it can be used as a green coffee extract (GCE). Abstract | Coffee contains high phenols, especially chlorogenic acid which acts as a modulation of the immune response, and antioxidants.
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